Deep Learning-Powered Cas13d Guide Design

Built from the largest Cas13d knockdown dataset to date, Arc‘s Cas13d Guide RNA Selection Tool nominates high-efficiency guide sequences for knocking down your transcript of interest across human, mouse, other model organisms, and any custom RNA sequences.
Wei et al,. Cell Systems, 2023

Explore the code and documentation on GitHub.

Recommended guide RNAs for targeting Human transcripts (based on Refseq GRCh38)

To find the top 50 recommended guides sorted by predicted efficiency for your transcript of interest, please use the search interface below.

To access the whole data file containing guide efficiency and information for all possible guides for all genes, please visit "Human_all" and download the files.

This should match the RefSeq gene name.