Lizard Wizard & Wizard's Staff: Comprehensive Calcium Imaging Analysis Pipeline

Lizard Wizard and Wizard's Staff form an integrated solution for calcium imaging analysis, providing a comprehensive workflow from raw imaging data to detailed calcium activity analysis. Lizard Wizard is a Nextflow-based pipeline that handles the initial processing of raw calcium imaging datasets, through automated cell segmentation and signal extraction. Wizard's Staff then takes over offering Biologists sophisticated analysis of the extracted signals from their images, offering everything from pairwise correlation analysis to k-means clustering and detailed metrics for neural activity characterization.

Tool Features

End-to-End Processing

Complete workflow from raw calcium imaging data to publication-ready figures and datasets

Advanced Metrics Analysis

Extract rise time, FWHM, and Firing Rate Per Minute (FRPM) metrics

Correlation Analysis

Perform pairwise correlation analysis within and between neural populations

Spatial Visualization

Generate spatial activity maps and cluster visualizations

Automated Quality Control

Built-in validation and parameter optimization