Lizard Wizard and Wizard's Staff form an integrated solution for calcium imaging analysis, providing a comprehensive workflow from raw imaging data to detailed calcium activity analysis. Lizard Wizard is a Nextflow-based pipeline that handles the initial processing of raw calcium imaging datasets, through automated cell segmentation and signal extraction. Wizard's Staff then takes over offering Biologists sophisticated analysis of the extracted signals from their images, offering everything from pairwise correlation analysis to k-means clustering and detailed metrics for neural activity characterization.
Complete workflow from raw calcium imaging data to publication-ready figures and datasets
Extract rise time, FWHM, and Firing Rate Per Minute (FRPM) metrics
Perform pairwise correlation analysis within and between neural populations
Generate spatial activity maps and cluster visualizations
Built-in validation and parameter optimization