iAnalyzer

iAnalyzer is an analytical pipeline designed for the robust analysis of CRISPR screens. It streamlines the processing of CRISPR screening data, facilitating tasks such as quality control, alignment, and statistical analysis to identify significant genetic perturbations.

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Tool Features

Comprehensive Analysis Pipeline

Handles the entire workflow from raw data processing to result interpretation

Flexible Metadata Handling

Utilizes a metadata file to specify sample information, enhancing reproducibility and organization

Support for UMI Processing

Offers options to process data with or without Unique Molecular Identifiers (UMIs), accommodating various experimental designs

Customizable Alignment Options

Currently supports Bowtie2 for sequence alignment, with provisions for integrating other aligners

Statistical Weighting Methods

Provides multiple options for combining z-scores, including sample size ('n'), standard error ('SE'), and standardized effect size ('SES')